FEASIBILITY STUDY OF PREPARATION OF CO-AGGLUTINATION COMPLEX FOR RAPID DETECTION OF VIBRIO CHOLERA IN VACCINE RESEARCH AND LABORATORY BY USING STAPHYLOCOCCUS AUREUS PROTEIN A

Background and Aim:Vibrio cholera is a gram-negative bacterial pathogen that causes lethal diarrheal disease cholera. From the first attempting to produce vaccine and in continue up to now a rapid method to recognition of vibrio were required. In this study, we try to present a rapid test to detection of vibrio cholera in laboratory and vibrio vaccine process and in process quality control in biotechnology or food manufactories .Methods:New Zealand White rabbits were first immunized with Vibrio cholera Ogawa and Inaba whole cell. Mono-specific gamma globulin was obtained, purified and concentrated by ammonium sulfate precipitation method. These antibodies were fixed on Staphylococcus aureus Cowan 1 NCTC-8325 whole cell. Rectal swab sample were inoculated into an enriched Alkaline Peptone Water medium pH 8.6 incubated for 5 h at 37 °C. One drop of each sample was mixed to with a drop of vibrio cholerae mono-specific co-agglutination reagent and results read in 2-3 minutes When 35 specimens were tested, the sensitivity and specificity compared with standard culture methods, were 93.33 % and 100%, respectively.Results:co-agglutination test is a simple, rapid, and reliable method to detect Vibrio cholerae in fecal specimens would assist in the management of cases of severe diarrhea, especially since most such cases occur in areas with minimal laboratory facilities.Conclusion:the results show that the co-agglutination method is sensitive, specific, easy, fast and inexpensive method for diagnosis of vibrio cholera, which is available with low-availability laboratory equipment. It is also an appropriate method to control and diagnose cholera.

کلیدواژه ها:

Complex ، Protein A ، Staphylococcus aurous ، vibrio cholera ، Co agglutination