EFFECT OF ULTRASOUND ON CYANOTOXIN DESTRUCTION

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 372

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شناسه ملی سند علمی:

MEDISM19_283

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Cyanobacteria are photosynthetic organisms with a very simple structure. Although, most of the cyanobacterial groups have capability to produce toxins and the toxins has destructive effect on living organisms, therefore, the present study try to screen cyanobacteria with ability to produce toxins and then evaluate effect of ultrasound on the same.Methods:30 water samples have been collected Kor River. Then serially diluted and cultivated on BG 11 Agar under light and dark conditions .The colonies were analyzed using microscopic and molecular identification by specific primers for the toxins. Then ultrasonic transducer operated at 23-48 kHz equipped with a 40 mL reaction vessel which is fitted with a Teflon window. The reaction vessel was centered at a distance of 4.50 cm . Since modest heating is observed during ultrasonic irradiation, the entire assembly was submerged in an ice bath to maintain a constant temperature of 4 C throughout the reaction process. Then the toxins destruction was monitored by HPLC with photodiode array detectorResults:The results indicated that out of 4 isolates 3 were produced the microcystin and 2 were produced cylindrospermopsin. The isolates were belong to: Geminocystis ، Cyanobacterium sp. KSU-AQIQ-3 ، Synechocystis aquatilis and Cyanobacterium stanieri The degradation of the extracted toxins was confirmed by HPLCConclusion:Although cyanotoxins are important for human health the ultrasound could be effective for destruction of the toxins.

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نویسندگان

Nakisa Nikoee

Dept. Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Shiraz, Iran

Nima Bahador

Dept. Microbiology, College of Science, Agriculture and Modern Technology, Shiraz Branch, Shiraz, Iran