ISOLATION AND CHARACTERIZATION OF PHOSPHORUS SOLUBILIZING BACTERIA

Background and Aim:Phosphorus is one of the vital elements for living and growth of all plants. Due to deficiency of the available soil phosphorus to plants, utilization of phosphorus fertilizers is an inescapably Necessity. Perilous side effects of continuity usage of chemical fertilizers are undeniable. Phosphorus solubilizing bacteria are able to disposal phosphorus that was trapped in the soil to plants. Therefore; the application of this bacteria as bio-fertilizer can be the best solution to reduce or stop the high usage of chemical fertilizers.Methods:Samples of the soil were collected from different agricultural lands in bagharz. Bacteria of the soil were isolated by serial dilution method. These bacteria were classified according to the physicochemical tests. To determination of the phosphorus solubilizing bacteria, the YED-P medium was used. The Bacteria with forming big halo zone on the YED-P medium were chosen. To the selection of the most appropriate bacteria, pH and thermal tests were done.Results:At first, 108 bacteria were separated. Thirteen bacteria were able to form a clear zone on the YED-P medium. Two of the thirteen bacteria that capable to make a big clear zone was chosen, and then pH and thermal test were done to them. Results showed that selected bacteria had good stability in different pH and thermals conditions.Conclusion:Phosphorus solubilizing bacteria can be the best option that we have natural agriculture free of chemical materials. They are able to provide P to the plants in the absorbable forms without any toxicity.

کلیدواژه ها:

natural agriculture ، bio-fertilizer ، soil bacteria