OPTIMIZATION OF MEDIA AND CULTURE CONDITION OF PSEUDOMONAS BACTERIA TO INCREASING THE DEGRADATION OF POLYETHYLENE

Background and Aim:Today، growing of plastic consumption in iran is over 2 million tons in each year .Therfore,it cause differnt problems in the environment.Thus,the purpose of current study was to optimization of media and culture condition of pseudomonas bacteria to increase the degradation of polyethylene as one of the main components in plastic products.Methods: The standard strain of pseudomonas aeruginosa (PTCC 1562) was grown in 100 ml of the inorganic medium (based on the McFarland standard).After adding 100 mg of low-density polyethylene nylon slices (2cm.2cm),culture incubated for 10 days and then the slices was washed by SDS .Finally, lost weight was measured and the culture condition was optimized.Results:Our results indicated that Polyethylene was degradaded by 2%in the initial conditions by the standarad bacterial strain .Also the highest polyethylene breakdown(PH7)was 2% weight loss at a temperature of 30C,3.4% at 30 days incubation time,4.4 % at 120rpm of shaker,2.7% in the presence of yeast extract with an 3.8% estimated weight loss.Finally, the removal of polyethylene in set of optimized conditions by pseudomonas aeruginosa PTCC1562 was 7.4%.Conclusion: The result of this study showed that this effective strain can be used for removal of polyethylene.

کلیدواژه ها:

polyethylene ، pseudomonas aeruginosa ، plastic biodegradation