EFFECT OF UV RADIATION ON THE SACCHAROMYCES CEREVISIAE FOR IMPROVING ITS TOLERANCE AGAINST 1- BUTANOL

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 496

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شناسه ملی سند علمی:

MEDISM19_325

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim: Butanol is a class of chemicals, which has several applications such as fuel, industrial solvent, etc. Saccharomyces cerevisiae, in particular, is a well-studied organism with a long history of industrial use; e.g. in brewing and ethanol production. Different isomers of butanol exhibit different extents of toxicity. 1-butanol being the most toxic with concentrations above 1.5-2% (v/v), is inhibitory for most cells including the native 1-butanol producers. One of the reasons for this is probably that 1-butanol is a hydrophobic molecule with the strongest ability to permeate and/or interact with the cellular membrane components. Random mutagenesis by physical mutagens such as UV radiation seems to be an uncomplicated method for yeast strain improvement.Methods: 0.1 ml of a suspension of about 1.7× 108 cells/ml of industrial strain Ethanol red of Saccharomyces cerevisiae was plated on YEPD-1-butanol agar medium, containing (g/l): yeast extract,10; peptone, 20; dextrose, 20; 1-butanol, 2%, 2.25% and 2.5% (v/v); agar, 20; pH 5.5. The plates were exposed to short wavelength U.V. light, (280 nm) from a distance of 20cm by using a 30W germicidal U.V. lamp in different times up to 180 seconds, until a survival of about 0.01% was obtained.Results:Mutants that were resistant against 1-butanol (2%, 2.25% and 2.5%) were selected.Conclusion:In this research, the procedures of creating a novel mutant of Saccharomyces cerevisiae which was screened from a mutated population of yeast, which was treated by UV light and screened in different concentration of 1-butanol is described.

نویسندگان

Fatemeh Sheikhi

Sugarcane Training and Research Institute of Khuzestan

Khosrow Rostami

Iranian Research Organization for Science and Technology

Mehrdad Zain

Iranian Research Organization for Science and Technology

Mohammad Ali Asadollahi

Industrial Biotechnology, University of Isfahan