STUDYING THE ANTIBACTERIAL PROPERTIES OF CHITOSAN NANOPARTICLES ALONG WITH ORIGANUM ESSENTIAL OIL TO INCRESE THE NEPHROPIDAE SHELF LIFE AT REFRIGERATOR TEMPERATURE

Background and Aim:Along with the increasing growth of Nanotechnology use in producing antimicrobials and improving properties of nanoscale comparing with raw materials,the need to use this new knowledge is felt in order to increasing the shelf-life of food products that are prone to corruption.Methods:The purpose of this research is to study antimicrobial properties of chitosan nanoparticles along with the Origanum essential oil to increase the shelf life of shrimp at refrigerator temperature. In this research the essential oil with concentration of 1&2% and nanoparticles of chitosan with concentration of 2% which is provided by the EPI method, are used.The samples are evaluated in 6 different treatments during a 14-day period in terms of microbial(Total Bacteria Count, Coliform and Staphylococcus Aureus)and chemical properties(TVN,TBA,PH)and sensory evaluation.Results:The results show meaningful difference between control sample and treatments in terms of Total Bacteria Count,Coliform and Staphylococcus Aureus(P<0.05).Two treatments which involve nanoparticles of chitosan along with concentrations of 1&2% of essential oil,have better microbial and chemical factors among the others.Conclusion:Although these coatings are able to meaningfully decrease the amount of Staphylococcus Aureus and Coliform colonies,the highest decrease is observed in Total Count on the fourth day.This means that the limitation of storing samples in the refrigerator in terms of microbial and chemical factors shouldn’t be more than 5days.The sensory evaluation also shows that there is a meaningful difference between the control and treated samples and the treatment involving a concentration of 1%of chitosan nanoparticles and 1% essential oil has scored more than the others.

کلیدواژه ها:

Chitosan nanoparticles ، Nephropidae ، Shelf life ، Origanum essential oil ، Antimicrobial properties