THE EFFECT OF MAGNETIC NANOPARTICLES ON THE GROWTH OF LUMINESCENT BACTERIA

Background and Aim:Magnetic nanoparticles are one of the most important nanoparticles that have been identified in microbiology by their effects on growth, morphology, and the yield of different bacteria. From 1995 to 2013, the EPA (U.S. Environmental Protection Agency) confirmed the performance of nearly 500 innovative environmental technologies for measuring water pollution. Exposure time for specimens varies from 5 minutes to 2 hours. One of these methods is the use of bioluminescence bacteria as biological markers that date back to the 1950s. Therefore, optimizing of these bacteria is very important for the measurement of water pollution. One of the optimization methods is use of Fe3O4 magnetic nanoparticles.Methods:Two strains of Vibrio Fischeri and Vibrio Harvey cultured in a swa medium with different concentrations of 20 nm nanoparticles of nano-us (50,100,150 and 200 μg / ml) at 25 ° C and 200 rpm. The growth rate and light emission of bacteria was measured using spectrophotometer and luminometer in 2 hours intervals, respectively.Results:Results The data was shown that light emission of V.fischeri and V.harvey in two different concentration of 150 microgram per ml and 50 microgram per ml, have positive effects on growth rate and cause more stability of light from bioluminescence activity, respectively.Conclusion:Optimizing luminescence bacteria improves the efficiency of measuring water pollution. Therefore, more precise measuring kits can be designed.

کلیدواژه ها:

luminescent ، optimization ، Vibrio ، light emission