OPRI MODIFIED GENE CLONING FROM PSEUDOMONAS AERUGINOSA BACTERIA IN E.COLI

Background and Aim:The Pseudomonas aeruginosa bacteria has a lots of disease factors and because of these factors creates infection in many parts of the body. Such as in lungs, burned-wounds and case of hospital infection. On the other hand, the resistance to antibiotic drugs is increasing. Perhaps, choosing the right vaccine can solve the problem. So, we suggest other membrane protein I (OprI) modified.Methods:Bioinformatics studies were performed on Pseudomonas aeruginosa strain PAO1. Extraction of oprI gene from Pseudomonas aeruginosa PAO1, primer design, PCR and cloning of this gene in the plasmid pET 28a and its transfer to Escherichia coli BL21 were performed. Then, using three confirmatory methods including universal primer PCR and enzymatic digestion.Results:In this studies with bioinformatics software, High conservation and strong antigenic propertis of OprI protein was demonstrated in Pseudomonas aeruginosa strains. In the laboratory experiments, the oprI was cloned in Escherichia coli BL21. And approval tests were positive.Conclusion:Cloning ability of this gene is suitable for the evaluation of the target vaccine.

کلیدواژه ها:

Pseudomonas aeruginosa ، oprI ، vaccine ، Cloning