ASSESSMENT OF ORNITHOBACTERIUM RHINOTRACHEALE INFECTION IN COMMERCIAL CHICKENS SUBMITTED TO POULTRY CLINIC OF RAZI INSTITUTE AND SLAUGHTERHOUSES OF KARAJ CITY

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 421

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شناسه ملی سند علمی:

MEDISM19_415

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Ornithobacterium rhinotracheale (ORT) has been associated with other respiratory pathogens in poultry and turkeys, and although the pathogenicity of the bacterium has been proven to be primary, but in most cases, along with other pathogens, it causes severe economic damage and causes respiratory symptoms, decreased growth, decreased Egg production and increased mortality and elimination of slaughter. The best vaccine is an autogenous vaccine prepared from local strains, and on the other hand, the latest isolates are required for research work.Methods:for bacterial isolation, after sampling from 60 herds at slaughter age. Samples were cultured in Blood agar containing gentamicin for 24h in 37°C at 5% CO2 then suspicious colonies examined by Gram staining for Pleomorphic and Gram negative bacterium. Confirmatory identification followed by biochemical test، including determination of oxidase (most strains Oxidase positive and probable oxidase-negative strains) and catalase reaction (Negation). The samples that were identified as ORT were subjected to DNA extraction of bacteria using standard phenol chloroform method. Then, a pair of specific primers to partial 16s RNA genome amplification fragment of 784 bp, used in PCR test.Results:About 20 (33%) strains were isolated in this research, in comparison to other studies in Iran the prevalence of ORT still is above according to European countries.Conclusion:so conclusively need more emphasis to implementing the hygienic measurements in poultry rising industries.

نویسندگان

Seyedgholamreza Mirzaei

Department of Avian Diseases, Research & Diagnosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran

M Banani

Department of Avian Diseases, Research & Diagnosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran

A Nouri

Department of Avian Diseases, Research & Diagnosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran