OPTIMIZATION OF BIOLUMINESCENCE OF VIBRIO FISCHERI

Background and Aim:Vibrio fischeri is bacteria that produce light, mainly in seawater, marine sediments, decomposing fish, and in the intestines of marine animals. The glowing light from luminous bacteria is a specific function consist luciferase and luciferin. This bacteria regulate their conserved lux genes by the mechanism of quorum sensing with produce specific substance auto-inducer (3-oxohexanoyl-homoserine lactone), during their growth, finally induced luciferase to emit visible light. This ability of V. fischeri is useful application for researchers and laboratory tests. In this study, we want to optimization growth condition for best emitting light.Methods:After all , we selected some factors can effect on bacterial growth like, temperature and culture medium ( NaCl concentration and Arg and Cys amino acid ). Sea water medium (5 ml) with 5 µl of V. fischeri incubated in 15, 18, 21, 24 ˚C and Room Temperature by shaking. RLU investigated in all of temperature by luminometer. For study of culture medium three concentration of NaCl (1%, 2%, 3% ) and Asp and Cys ( 0/005 , 0/004 gr)was used.Results:The result showed, light emitting of marine bacteria occurred in temperature of RT and 24˚C. Effects of NaCl displayed 1% NaCl and 0/005 Asp + 0/005 Cys are best concentration. Top RLU for this optimization was 24milion.Conclusion:according to this result optimized condition for best growth and shedding light achieved. We can used these optimized V. fischeri for examination water and food toxicity(microtox).

کلیدواژه ها:

V. fischeri ، luminous ، optimization