INVESTIGATION OF MUTAGENIC AND ANTI MUTAGENIC EFFECTS OF PORTULACA OLERACEA ON SALMONELLA TYPHIMORIUM TA100 USING AMES METHOD

Background and Aim:The purpose of this study is to investigate the mutagenic and anti-mutagenic effects of the extract of the plant portulaca oleracea on salmonella typhimorium(TA100) and determine its effective dose. Since it is simply not possible to test these materials for eukaryotic cells, Professor Ames and his colleagues presented a low-cost, short-term, and easy bacterial test to find out the effects of mutagen substances.Methods:In this test, genetically manipulated strains of salmonella typhimurium are used. These strains are converted into wild straw by contact with mutagenic materials. Histidine mutants with probable mutagenic substances (the extract of the plant of porulaca oleracea) are mixed on a MGA plate with some histidine and biotin; after 48 hours of incubation, the colonies are counted. due to absence of the CYT P450 enzyme system in the bacteria, rat liver enzyme is used as a buffer S9Results:Results show that portulaca oleracea does not have any mutagenic effects on salmonella typhimorium(TA100) with or without metabolic activation, but antimutagenic effect is observed in methanolic,hydroalcholic and aqueos extracts at dilution of 71-142-258 µg/ml without metabolic activation.Conclusion:it does not have mutagenic effect but it has anti mutagenic effect at 3 dilition of hydroalcholic and aqueos without metabolic activation.