MOLECULAR IDENTIFICATION OF PAPILLOMAVIRUS TYPE 16 AND 18 ISOLATED FROM WOMEN WITH CERVICAL CANCER

Background and Aim:Women with human papillomavirus (HPV)-associated with cervical and breast cancer have a higher mortality than the general female population. The purpose of this study was to identification HPV-16 and HPV-18 genotypes in patients with cervical cancer or breast cancers by multiplex-PCRMethods:. In this experimental study, after collecting of samples from malignant cervical cancer, the viral DNA was extracted by SinaClon kit and PCR was done by specific primers for HPV-16 and HPV-18 gene of human papillomavirus in all samplesResults:After the analysis of PCR products by 2% agarose gel electrophoresis. Among 60 patient samples, 19 cases were confirmed to be positive for HPV infection and 41 cases were negative, showing high frequency of HPV in this patient population (about 31.6%). The frequncy of HPV-16 and HPV-18 were 8(1/42%) and 11 (9/57%) cases, respectivelyConclusion:This study showed that PCR by specific primers for HPV-16 and HPV-18 gene of human papilloma virus is a proper and accurate method for detection of this virus and the results confirm the previous reports of correlation between HPV and cancer samples.

کلیدواژه ها:

Cervix cancer ، Human Papilloma Virus ، M-PCR