STUDY OF SIRT3 EXPRESSION IN PATIENTS SUFFERING FROM GASTRIC CANCER AND HELICOBACTER PYLORI INFECTION SIMULTANEOUSLY

Background and Aim:Gastric cancer (GC) is one of the most common malignancies with high rates of mortality worldwide. Although incidence of GC is decreasing, it is still a major clinical challenge because of delay in diagnosis, poor prognosis and limited treatment options. Helicobacter Pylori (H.Pylori) is a major cause of peptic ulcer disease and gastric malignancies. Sirt3 may function as an oncogene in different cancers. In this study, we evaluated the expression levels of Sirt3 gene in GC tissue biopsies.Methods:A total of 44 tissue biopsies of gastric cancer (25 patients with positive test result for H.pylori infection versus 19 ones with negative result) were enrolled in this study. Urease test was used for detection of H.Pylori infection in tissue samples. RNA Extraction of tissue biopsies were performed via RNeasy Mini Kit (QIAGEN-Germany). Expression of Sirt3 was assayed by qRT-PCR using Taq-Man probes and specific primers.Results:Expression of Sirt3 in H.Pylori positive patients group with gastric cancer significantly increased in comparison with H.pylori negative patients (P value=0.001) There was no significant relation between gender, age and expression of Sirt3 (P value=0.65 and 0.33 respectively).Conclusion:Sirt3 expression increased in H.Pylori positive tissue samples of gastric cancer and seems a promising biomarker in diagnosis and prognosis of patients suffering from gastric cancer and H.Pylori infection simultaneously.

کلیدواژه ها:

Sirt3 ، Gastric cancer ، Helicobacter Pylori