STRUCTURAL AND FUNCTIONAL DESIGN AND EVALUATION OF NEW IMMUNOTOXIN STRUCTURES AFFECTING LIVER CANCER IN QUASI-PHYSIOLOGICAL CONDITIONS

Background and Aim:Introduction:Liver cancer is the fifth most common cancer in the world and the second leading cause of death due to cancer in men,Treatments such as surgery, radiation therapy,chemotherapy and liver transplantation are commonly treated.The side effects of these therapies and their inefficiencies have led to the development of strategies for the targeted treatment of cancer cells based on their molecular variations,such as the antigen of cell surface called immunotoxin.Methods:Detection of specific antigens on the surface of cancer cells and the components that make up these types of drugs and their assembly is a major step in designing these types of drugs,Which was based on computational science related to liver cancer.The results of this research.Results:Results Has led to the detection of 18 antigenic expressions at the level of liver cancer cells with the highest and most specific expression associated with ANO7,CACNA1A and AFP antigens, In terms of expression, ANO7was selected as the best antigen.Among the protein molecules that can be attached to this antigen,KLK2was selected as the most effective ligand.The assimilation of Pseudomonas aeruginosus toxin-derived and selective ligand by linker (GGGGGG) resulted in the creation of five recombinant structures of varying quality and structure,One of the structures showed the desirable quality of structural and functional stability after being placed in real-world conditions.Conclusion:The results of this research led to the introduction ANO7 antigen as an appropriate choice In the goals of effective immunotoxin drugs against liver cancer,which should be laboratory tests.

کلیدواژه ها:

Immunotoxin ، Toxin ، Ligand ، Antigen ، ANO7 and KLK2