ASSESSMENT OF FREQUENCY AND NUCLEOTIDE SEQUENCE OF IRON RECEPTOR PMI0842 IN PROTEUS MIRABILIS COLLECTED FROM URINARY TRACT INFECTIONS CASES IN TEHRAN, IRAN

Background and Aim:Proteus mirabilis strains are among the most important causes of Urinary tract infections (UTI) especially in complicated UTIs. Increasing rate of antibiotic resistance will complicate future treatment of UTIs, making the development of a vaccine more urgent. There is no study about the evaluation of some iron adsorption systems of Proteus mirabilis as vaccine candidates. Thus, in this study we evaluated the presence and nucleotide sequence of iron receptor PMI0842 in Proteus mirabilis collected from UTIs cases in Tehran, Iran.Methods:Totally, 120 Proteus mirabilis isolates were collected from UTI patients in Tehran, Iran. Bacterial identification was performed by biochemical tests. Polymerase Chain Reaction (PCR) was applied to amplify the PMI0842 gene in the isolates. Then, the amplified PCR products of 15 isolates were purified from the agarose gel and were subjected to sequencing by designed primers. After sequencing, the sequences of these genes were compared with the genes deposited in GenBank and Expasy.Results:The PMI0842 gene was present in 80% of the Proteus mirabilis isolates tested. According to the results of Blast in NCBI, comparison of the nucleotide sequence of these sequenced genes with sequences deposited in Gen banks showed high similarity between these sequences.Conclusion:Our findings confirmed for the first time the conservation of PMI0842 gene among the Proteus mirabilis isolated from UTI patients. Thus, PMI0842 gene could be an ideal vaccine candidate against UTIs caused by Proteus mirabilis. Evaluation of efficacy of this iron adsorption receptor in vivo and in vitro is under study.

کلیدواژه ها:

Urinary tract infection ، Proteus mirabilis ، Iron receptors ، PMI0842