PREVALENCE OF RESISTANCE TO FLUOROQUINOLONES ESCHERCHIA COLI QNR GENES ISOLATED FROM PATIENTS ADMITTED TO JAHROM´S HOSPITALS BY PHENOTYPIC AND MOLECULAR METHODS, 2016-2017

Background and Aim: Quinolones are considered as antibiotics which play a key role in the treatment of ureteral, digestive, respiratory infections and septicemia and gonorrhoea as well. Excessive use of these antibiotics has raised resistance through these antibiotics; because of the presence of quinolones-resistance genes which are carried by plasmids. The present study was done to determine the frequency of quinolones-resistance in quinolones-producing Escherichia coli bacteria, using phenotypic and molecular methods.Methods:The number of 446 Escherichia coli samples were gathered from patients referrer to Jahrom health centres from spring 2016 to autumn 2017 and were surveyed by MR, VP, SIM, TSI, Urease and citrate diagnostic methods. To specify the phenotypic antibiotic resistance pattern, disc diffusion method was used and positive samples, after DNA extraction, were assayed by the molecular method to identify the quinolones genes in positive samplesResults: Disk diffusion results showed that the maximum resistance frequency belonged to Cefotaxim 184(41%), respectively. In addition, the least resistance was seen in Meropenem 0%. PCR results of quinolones-resistance genes indicated, 12 (%2.7) samples for qnrA and 9(2%) samples for qnrB and 10(2.2%) samples were positive for qnr S genes. Among all samples, 8 samples had two genes simultaneously, there were 3 (0.7%) samples with qnrA & B, 3(0.7%) samples with qnrA&S and 2(0.4%) samples with qnrB&S simultaneously.Conclusion:The results of the present study show the frequency of quinolones-resistance genes in Escherichia coli bacteria. According to raising of the prevalence of quinolones-resistance Escherichia coli bacteria, the sensitive antibiogram before any therapy is suggested.

کلیدواژه ها:

Quinolones-antibiotic resistance ، Escherichia coli ، qnrA ، qnrB ، qnrS