HIGH INCIDENCE OF TOXIN AND BIOFILM GENES AMONG METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS STRAINS

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 362

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شناسه ملی سند علمی:

MEDISM19_564

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Methicillin-resistant S. aureus (MRSA) is one of the predominant bacteria that causes wound infections in burn patients. MRSA produces several virulence factors such as adhesin and biofilm, hemolysins, enterotoxins, and exfoliative toxins. Staphylococcal cassette chromosome mec (SCCmec) typing method used to study MRSA molecular epidemiology. Therefore, the aim of this study was to determine frequencies of genes encoding virulence and SCCmec types in a MRSA collection that obtained from the burn patients.Methods:S. aureus isolates were obtained from wound infection of burn patients. Isolates were identified to species level using standard biochemical methods. The mecA gene was targeted by PCR in order to identify isolate as MRSA. PCR assay was performed to determine of SCCmec types, biofilm and virulence genes.Results:Of 165 S. aureus isolates, 69% isolates were MRSA. SCCmec typing of these isolates produced three different SCCmec types. 53% isolates were identified as type IIIA, 17% as type V and 2% as type I. The prevalence of genes encoding virulence factors in MRSA isolates were hla (61%), hlb (44%), sea (23%) and seb (2%). The sec, eta, tst, pvl, genes were not detected in any of the MRSA isolates in this study. The most prevalent genes encoding biofilm was eno, found in 61.1% of isolates, followed by fib and icaA found in 48.1% and 38.8% of the isolates, respectively.Conclusion:The results of this study are indicating the high prevalence of MRSA and virulence factors among S. aureus isolated from burn wound infection.

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نویسندگان

Mitra Motallebi

Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran

Mohammad Emaneini

Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Fereshteh Jabalameli

Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran