MOLECULAR DIAGNOSIS OF HELICOBACTER PYLORI IN SEMEN OF INFERTILE MEN

Background and Aim:Bacterial infections of the genital system are common causes of infertility. One of the most important causes of male infertility is seminal and genital tract infections. In the meantime, a wide range of bacteria, in varying degrees, contribute to infertility in men. Helicobacter pylori may be involve in infertility. This bacterium is widespread and about half the world s population is infected with this bacterium, so timely diagnosis can be effective in treatment and prevention. Background and aim: Determination of the role of H. pylori in seminal semen of infertile men by PCR.Methods:100 samples of semen collected from Saram hospital and DNA Extracted from these specimens using Boiling/DNG_PLUS method. Optimized PCR test was performed on samples. PCR test evaluated from the respect sensitivity and specificity.Results:In this study, amplicon with the size of 294 bp, observed with agarose electrophoresis. In the specificity test, primers created only a band for Helicobacter pylori DNA. Of the 100 samples tested, only 10 samples (10%) were positive for amplification.Conclusion:According to the results of this study, Helicobacter pylori may be one of the bacterial agents that can be considered for male infertility, of course requires further studies. While PCR is suitable, quick and sensitive molecular technique for detection of Helicobacter pylori in infertile men.

کلیدواژه ها:

Helicobacter pylori ، PCR ، ، infertile men