PREVALENCE OF PAPC GENE IN UROPATHOGENIC E.COLI ISOLATES FROM URINARY TRACT INFECTIONS IN RASHT AND THEIR ANTIBIOTIC RESISTANCE PATTERN

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 374

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شناسه ملی سند علمی:

MEDISM19_572

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Escherichia coli (E. coli) is the most common cause of urinary tract infections (UTIs) in many developing countries. Fimbriae, including P-fimbriae, are one of the factors involved in the virulence of this bacterium that plays an important role in the pathogenesis of uropathogenic E.coli (UPEC). The aim of the present study was to investigate the frequency of papC virulence gene in E.coli isolated from urinary tract infections in Rasht.Methods:In this study, a total of 76 clinical gram-negative isolates were collected from patients with UTI in some Rasht hospitals. The isolates were examined using different biochemical tests. Antibiotic resistance pattern of isolates was also evaluated by disc diffusion method. Genomic DNA of isolates was extracted using TIAGEN kit and presence of papC virulence gene was determined by specific primers using PCR method and sequenced for confirmation.Results:Of the 76 isolates examined, 50 isolates were confirmed as E. coli. The results of the antibiogram test showed that most isolates were resistant to cefazolin (74%) and ceftazidime (64%) and were sensitive to gentamicin (68%) and chloramphenicol and kanamycin (52%). The results of investigating the frequency of papC gene in E. coli isolates showed that 45 isolates (90%) of the 50 isolates had the above gene.Conclusion:These results confirm the essential role of papC gene in the pathogenesis of uropathogenic E. coli strains (UPEC).

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نویسندگان

Zahra Askari

Student of MSc in Microbiology, Faculty of Basic science, Rasht branch, Islamic Azad University, Rasht, Iran

Seyedeh Tooba Shafighi

Assistant Professor, Faculty of Basic science, Rasht branch, Islamic Azad University, Rasht, Iran