QUORUM SENSING INHIBITION POTENTIAL OF RUBUS ULMIFOLIUS BLOSSOM EXTRACT IN VITRO AGROBACTERIUM TUMEFACIENS NTL/PZLR4

Background and Aim:Many bacteria specially Staphylococcus aureus utilize quorum sensing mechanism in order to coordinate their vital functions such as survival, motility, production of biofilm, pathogenicity factors, etc. wereas signaling system Quorum sensing has a role in controlling disease factor, destruction by herbal plant is a good goal for anti Quorum sensing therapy, without using antibiotics. In this study interfering effects of distinguished plant extract, Rubus Ulmifolius Blossom on bacterial quorum sensing of Agrobacterium tumefaciens NTL/PZLR4 was evaluated.Methods:Staphylococcus aureus was isolated from patients with dental implant infection. Rubus Ulmifolius Blossom plant specie were collected from the surrounding agricultural areas of JahromCity. The collected plants were extracted using three organic solvents, 96% ethanol. The anti-quorum sensing and anti biofim bioassays were then performed to depletion of violacein and find out their microtiter plate property respectively. Results: Based on the results, Rubus Ulmifolius Blossom extract possess meaningful anti-QS activity and anti biofilm.Results:Based on the results, Rubus Ulmifolius Blossom extract possess meaningful anti-QS activity and anti biofilm.Conclusion:Due to decrease in the production level of violacein by NTL/PZLR4 as a result of the anti-quorum sensing activity of Rubus Ulmifolius Blossom extract, and als the destraction of the power of producting the biofilm of Staphylococcus aureus application of the extract can be considered as an appropriate approach for controlling bacterial pathogens without developing resistance.

کلیدواژه ها:

Rubus Ulmifolius Blossom ، Staphylococcus aureus ، Inhibition ، Quorum sensing ، Agrobacterium tumefaciens NTL/PZLR4