ANTIMICROBIAL POTENTIAL OF TIO2 NANOPARTICLES AGAINST MDR PSEUDOMONAS AERUGINOSA

Background and Aim:Infections caused by multi-drug resistant (MDR) strains Pseudomonas aeruginosa are becoming extremely difficult to treat with conventional antibiotics, leading to a sharp rise in clinical complications. Along with the increase in the prevalence of resistant strains due to the abuse of antibacterial agents, there is an urgent need for a new class of antibacterial agents with an entirely different structure and mode of action. In recent years, various nanoparticles have been reported to display good biological activities. In the present study, the efficacy of TiO2 nanoparticles as an antibacterial agent against MDR P. aeruginosa was explored.Methods:Ten clinical MDR isolates of P. aeruginosa were obtained from Imam Reza Hospital, Mashhad. Antimicrobial susceptibility of all the P. aeruginosa isolates was performed according to the CLSI guidelines (CLSI, 2017). The optical property of TiO2 nanoparticles was studied by ultraviolet-visible (UV-vis) spectroscopy.Results:As mentioned before , bacterial strains identification was performed through Phenotyping and Genotyping. In identification step , 35 bacterial smaples with highest nanoparticle production were identified . Microbial strains identification As mentioned before , bacterial strains identification was performed through Phenotyping and Genotyping. In identification step , 10 bacterial smaples with highest nanoparticle production were identified .Conclusion:Obtained results from TEM confirmed the presence of Tio2 nanoparticles in microorganisms supernatant culture . Silver nanoparticles existed in culture as bacillus and dispersed which their sizes were from 20 to 400 nm.

کلیدواژه ها:

Titanium oxide ، S.aerjinosa bacterium ، bedsore ، antibiotic