COMPARISION OF SEROLOGICAL TESTS AND ELISA FOR DIAGNOSIS OF BRUCELLOSIS IN SHEEP AND GOATS

Background and Aim:Brucellosis is one of the most important zoonotic diseases which is endemic in Iran. The most widely used methods of diagnosis are based on serology. The aim of this study was to compare serological tests for detection of brucellosis by Rose Bengal test (RBT), Wright, 2-mercaptoethanol and ELISA tests in Khuzestan province.Methods:A total of 447 sera samples were collected and tested from sheep and goat herds during the time period of July 2017 to February 2018. 45 (10/06%) positive Rose Bengal test, Wright, 2-mercaptoethanol serum samples, ready for slaughter, and 402 negative serum samples were gathered and tested by commercial indirect ELISA ID vet and the results obtained were recorded.Results:The results showed that out of 45(10/06%) positive Rose Bengal, wright and 2ME samples and 402 negative ones, 179(40/04%) were positive according to ELISA ID vet kit.Conclusion:The results of this study showed that this ELISA kit is designed for European countries in which their control strategy is slaughter in sheep and goats with serology while in Iran, the strategies of disease control are vaccination, test, and slaughter. Therefore, it is recommended that this method is redesigned for practical use by changing the cut-off point of the commercial kit.

کلیدواژه ها:

Brucellosis ، diagnosis ، ELISA ، Wright test ، 2ME test