FIELD INVESTIGATION OF MYCOPLASMA GALLISEPTICUM INFECTION IN BACKYARD AND COMMERCIAL CHICKEN IN URMIA USING PCR METHOD

Background and Aim: Mycoplasma gallisepticum (MG) is the causative agent of chronic respiratory disease (CRD), with a significant economic impact on poultry farms. Asymptomatic infections can make birds susceptible to secondary infections.In field, diagnosis is mainly carried out based on the clinical symptoms by veterinarians. However, to confirm the diagnosis, robust laboratory detection of infection is necessary. Molecular detection using PCR is considered a sensitive method.Methods:To understand the prevalence of MG infection in the poultry carcases refered to veterinary clinics around Urmia, a PCR assay was developed using the gyrB gene. In total, 80 tracheal swabs from commercial and backyard chicken carcasses with clinical signs were collected and the extracted DNA were investigated using PCRResults:It had turned out that only 6 out of 50 commercial (12%) and 3 out of 20 backyard samples (15%), which were clinically diagnosed with MG infection found to be MG positive, suggesting that diagnosis made based on the clinical signs is not conclusive and final diagnosis needs to be confirmed by molecular diagnosis for accurate antimicrobial agents and vaccine administration.Conclusion: diagnosis made based on the clinical signs is not conclusive and final diagnosis needs to be confirmed by molecular diagnosis for accurate antimicrobial agents and vaccine administration

کلیدواژه ها:

Mycoplasma ، gallisepticum ، Molecular ، clinical signs