THE SPORE-FORMER PROBIOTICS REDUCE THE EFFECTS OF SALMONELLA ON THE GUT MICROFLORA POPULATION

Background and Aim:The intestinal lumen is a major way for pathogens entrance such as Salmonella. But in the gut lumen, the pathogens face a dense microbiota. This community confers health benefits. The probiotics cause the enhancement of colonization resistance and direct inhibitory effects against pathogens which are important in reduction the incidence and duration of gastroenteritis.Methods:In this study 60 rats were randomly divided into three groups (each group consists of 5 subgroups- 4 rats in each subgroup) as follows: Group 1: Control. Group 2 (Pro.+Sal.): Receiving water containing 5×107 spore/ml Bacillus subtilis and 5×107 spore/ml Bacillus coagulans for 29 days and intragastric gavage of 109 CFU of Salmonella Typhimurium at day 22. Group 3 (Salmonella): Receiving intragastric gavage of 109 CFU of Salmonella Typhimurium at day 22. At day 23, 25, 27 and 29 of the experiment, the total aerobic and anaerobic microorganisms, lactic acid bacteria (LAB) and Coliforms were counted in the feces samples in each subgroup.Results:Salmonella caused no change in aerobic bacteria enumeration, but in Pro.+Sal. group it reduced and returned to the normal range in 5 days. In Salmonella group anaerobic and Coliform count increased, but probiotic reduced these enhancements and the population were kept close to the control group values. Also, probiotics inhibited the reduction in LAB population following Salmonella infection.Conclusion:B. subtilis and B. coagulans help the maintenance of gut microflora population when expose to the pathogen, which, causes the reduction of pathogen effects and early recovery.

کلیدواژه ها:

Spore-forming probiotic ، Gut microflora ، Salmonella Typhimurium