Determination and comparison of antibacterial activity of Enterococcus species isolated from breast-fed neonates and adults feces in Kerman

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 403

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شناسه ملی سند علمی:

MEDISM20_016

تاریخ نمایه سازی: 26 بهمن 1398

چکیده مقاله:

Introduction and Objective: The genus Enterococcus, previously recognized as fecal stereptococcus, are microbiota of the intestine of human and other animals. They usually exerts anti-Listerial activities, in addition their presence in breast milk helps maintain the baby s health. This bacterium is one the first bacteria colonized in the neonate intestine and meconium, it could emphasize on its importance. In this study we aim to determine the antibacterial activity of Enterococcus spp. from fecal sample of neonates and healthy adults with comparison of their activities on 10 different standard bacterial strains. Material and Method: Totally, 87 Enterococcus strains were collected from feces samples of breast-fed neonates aged 3 to 7 days and healthy adults that had not used any antibiotics or probiotic products. Conventional methods were used for bacterial identification. Antibacterial testing was performed through spot test bilayer against staphylococcus aureus, ATCC25923 listeria monocytogenes CCUG 15527, Enterococcus Fecallis PTCC 1237, pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Enteropathogenic E. coli (EPEC) ATCC1269 bfp, E.coli O157.H7 NCTC 1290, Enterotoxigenic E. coli (ETEC) H10407, Enteroinvasive E. coli ( EIEC) 85b, klebsiella pneumoniae ATCC 70063. Data of antimicrobial effects against indicator pathogens were recorded according to the following scale: no inhibition zone (-), inhibition but no clear zone (1≥ , ±).weak inhibition zone (1 to 2, +).good inhibition zone (2 to 4, ++). high inhibition zone(4<, +++). Result: E.facium was the most commonly isolated species followed by E.fecallis. Many isolated exhibited inhibitory activities against pathogenic strains. More than 50% of the Enterococcus isolate had good and high antibacterial properties against L. monocytogenes, EPEC, E.coli, E.fecalis and P. aeruginosa. ETEC was the least inhibited pathogen. Enterococcus isolated from neonates had significantly higher antibacterial properties than those isolated from adults (p-value≤ 0.05). Conclusion: Enterococcus isolates have good and high inhibitory activity especially against L. monocytogenes and at a lower level against EPEC, E.coli, E.fecalis and p. aeruginosa. More in vitro and in vivo clinical data is needed before any conclusion on the probiotic properties of the isolates could be drawn.

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نویسندگان

Mryam Rahmani

Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Fereshteh Safari

Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Shahla Mansouri

Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran