Impact of culture media kind on alpha toxin production of Clostridium perfringens

Introduction and Objectives: Clostridium perfringens phospholipase C (PLC), commonly known as alpha toxin, is the lethal, dermonecrotic toxin produced by all strains and is considered a major virulence factor in clostridial myonecrosis. ELISAs have relatively high sensitivity and can detect small amounts of toxin in samples Materials and Methods: Three culture media, that are selected was liquid Thioglycolate medium, the base media of c. perfringes and also c. novyi vaccine that contain pepton and the other nutrients. 10 Strains of C. perfringens was cultured in these three culture medias and incubated at 37 C for 6 hours under anaerobic conditions.an enzyme-linked immunosorbent assay (ELISA) with antibodies specific to alpha toxins of C. perfringens was done on all Culture supernatants by using a Bio-X Alpha toxin ELISA kit. The results determined optical densities by means of a microplate spectrophotometer with a 450 nm filter Results: Interpreting the results show that the presence of toxin in all samples was positive but there were differences between ODs. Conclusion: the amount of alpha toxin produced by c. perfringens may be influenced by the kind of the media.

کلیدواژه ها:

c. perfringes ، culture medias ، alpha toxin ، ELISA