Lytic effect of specific bacteriophage cocktail against Salmonella (enteritidis, typhimurium, and infantis) in HEP2 cell line

Introduction and Objectives: Salmonellosis is one of the most important infectious diseases among humans and animals, and it has many problems in response to common antibiotic treatments. Today, due to the emergence of drug resistance to antibiotics, there should be a good alternative to drugs. Bacteriophages or phages for short, are viruses that attack bacteria and destroy them. Due to their specific properties, the lytic bacteriophages can be considered as an appropriate choice for the treatment of infectious diseases. Materials and Methods: Standard strains of Salmonella (enteritidis, typhimurium and infantis) were prepared from the Faculty of Veterinary Medicine, University of Tehran, and their specific bacteriophages were isolated using soft agar method. In the next step, the direct effect of each strain on the target cell line was investigated, and the effect of the inoculated bacteriophage was studied individually and finally as a cocktail on the strains mentioned. Results: Particular plaques of bacteriophages isolated from salmonella were specific to each salmonella, and had no lytic performance against other salmonella and other intestinal pathogenic bacteria. The binding and pathogenicity of Salmonella strains and cocktails prepared, as was expected, was positive after inoculation, as well as their lytic function, were observed. Conclusion: Overall, due to the increasing prevalence of antibiotic resistance among salmonella and concerns about their treatment, it is anticipated that in the future, these phages can well be used as a proposed for disease control and prevention, both in vivo and in vitro conditions.

کلیدواژه ها:

Salmonella ، Bacteriophage ، Salmonellosis ، HEP2 cell line