Inactivation of Acinetobacter baumannii from hospital wastewater using UV/H2O2 process as a new approach for oxidation: Modeling and optimization

Introduction and Objectives: Hospital wastewater by containing multiresistant bacteria and their resistance genes can represent a high biological risk for public health. Multidrug-resistant Acinetobacter baumannii (A.baumannii) is an emerging pathogen mostly reported as a cause of hospital infections. Because of its high repetition of antibiotic resistance and escaping the biocidal ability of antibiotics, A.baumannii emphasized among the ESKAPE pathogens. In the current work, the UV/H2O2 process applied for efficient inactivation of A.baumannii from hospital wastewater. Materials and Methods: The central composite design used to enhance the inactivation efficiency of A.baumannii by response surface methodology (RSM). The experiments were done under four levels of various operational parameters. The influence of operating parameters such as the initial concentration of A.baumannii concentration, H2O2 dosage, UV intensity, and reaction time investigated using response surface methodology. The isolated A.baumannii determined CHROM agar Acinetobacter and several antibiotic discs. Results: The present study focused on the inactivation of A.baumannii from hospital wastewater. The obtained results revealed that the UV/H2O2 process was able to inactivation A.baumannii effectively. Regression analysis revealed a satisfactory agreement between the obtained experimental results and the predicted inactivation efficiency by the second-order polynomial model. The kinetics of the process follows the pseudo-first-order model. Conclusion: The UV/H2O2 process applied successfully for A.baumannii inactivation from hospital wastewater and the predicted model for treatment of synthetic wastewater is in satisfactory agreement with inactivation efficiency of real hospital wastewater treatment.

کلیدواژه ها:

Inactivation ، Acinetobacter baumannii ، UV/H2O2 process ، Hospital wastewater ، multiresistant bacteria.