Isolation, morphological characterization and cross-host determination of lytic phages infecting multidrug resistant Pseudomonas aeruginosa from different sources

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 283

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شناسه ملی سند علمی:

MEDISM20_318

تاریخ نمایه سازی: 26 بهمن 1398

چکیده مقاله:

Introduction and objectives: Pseudomonas aeruginosa colonization is one of the major complications of burn wound infection leading to higher risk of morbidity and mortality. Unfortunately, antibiotic resistance among Pseudomonas aeruginosa is increasing due to irregular and overuse of antibiotics. Phage therapy, eradicating of infecting bacteria with bacteriophages,could be a future alternative to antibiotic treatment of bacterial infections. This study was aimed to isolate bacteriophages against MDR-Pseudomonas aeruginosa isolated from burn wounds from hospital sewage, soil, and sewage polluted rivers in Iran and determination of their cross-host by EOP.Material and Methods: Samples were collected from hospital sewage, soil and polluted rivers in Rasht and Hamadan four times. Suspensions of various strains of Pseudomonas aeruginosa isolated from burn wounds were mixed with samples in double LB broth. Lytic phages isolated after centrifugation, filtration and serial dilution. Finally, host range against 12 MDR-Pseudomonas aeruginosa were determined by EOP to evaluate the effect spectrum of the bacteriophages. Furthermore, morphological characteristics were determined using TEM (Transmission electron microscopy). Results: In this study 18 lytic bacteriophage were isolated after four times sampling . 5,9 and 4 phages isolated from sewage, polluted rivers and soil respectively. Six of eighteen phages were selected as highly effective phages that infect wide range of burn wound infecting MDR-Pseudomonas aeruginosa by EOP. TEM imaging revealed all isolated phages belonged to Myoviridae family. Conclusion: EOP showed that more Pseudomonas aeruginosa strains were lysed by phages isolated from soil compared to other sources.

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نویسندگان

Bahareh Lashtoo Aghaee

Microbiology Department, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Ali Mojtahedi

Microbiology Department, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Mohammad ali Khanmirzaii

Microbiology and Immunology Department, Duff Medical Building, McGill University, Montreal, Canada.

Mohammad Shenagari

Microbiology Department, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Mohammad Yousef Alikhani

Microbiology Department, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.