Synthesis of a quercetin-loaded hydrogel based on Hyaluronic Acid-Polydimethylsiloxane (HA-PDMS) with antibacterial activity

Introduction and Objectives: One of the recent applications of the hydrogels is wound dressing to promote the wound healing process. In this study we synthesized a quercetin-loaded HA-PDMS composite hydrogel in which quercetin antibacterial activity was stabilized. Materials and Methods: Cross-linked HA hydrogel was synthesized using PDMS-DG in alkaline condition, for 2 hours at 37 °C. Cross-link between HA and PDMS was performed based on OH (from HA) and epoxy (from PDMS) reaction leading to ether bond formation. After that, the room temperature (RT) dried hydrogel was immersed in quercetin solution (4 or 2 mg/mL) for 24 hours in dark and RT. Quercetin-loaded hydrogel was characterized using NMR analysis. Antibacterial activity of the quercetin-loaded hydrogels was investigated against Psudomonas aeruginosa PAO1 using disk diffusion and bactericidal efficiency (40 mg of quercetin-loaded hydrogels was dissolved in a 1ml medium. Then 150 μL mixture of bacterial suspension at 106 CFU/ml and hydrogel solution (1:1) was cultured for 24 hours at 37 °C. Results: HA-PDMS hydrogel was synthesized as a transparent gel with vial inversion property. Loading with quercetin, changed the color of the hydrogel to yellow. NMR analysis showed that the HA-PDMS hydrogel and quercetin-loaded hydrogels were synthesized successfully. According to disk diffusion test on Mueller Hinton agar, 12 mm inhibition zone around the quercetin-loaded hydrogels was observed against P. aeruginosa. Also, bactericidal efficiency test revealed 66.76% and 15.75% inhibition in P. aeruginosa growth in the case of 4 and 2 mg/mL quercetin immersed hydrogels, respectively. Conclusion: Based on our results, quercetin-loaded hydrogel has a good potential in bacterial infections and wound healing management.

کلیدواژه ها:

Hyaluronic acid ، PDMS-DG ، Quercetin ، Antibacterial hydrogel