Phylogeny and characterization Of Iranian Fowl Adenoviruses based on the penton gene, 2019

Introduction and objective: Since 2012 Inclusion Body Hepatitis (IBH) has happened as an economically important disease in Iran and the number of IBH cases has been increased. Disease consequences and induced sudden deaths threaten poultry industry; thus, characterization of species and serotypes is helpful to implement preventive strategies. Material and Methods: PCR is a quick and appropriate method for identification and characterization of IBH virus. IBH was routinely recognized based on parts of the hexon gene, but in this survey, the penton gene has been studied for the first time in Iran. Six viruses were identified from broilers of north, west, and center of Iran during 2019. Sequence alignment and phylogenetic analysis based on 1406 bp region of the penton gene were done. Results: Results indicated that detected viruses belonged to species D mostly serotype 11. Conclusion: Similar results about cluster patterns obtained in the current study with those from previous studies, supported the hypothesis that the penton gene can be replaced with the hexon gene in phylogenetic analyses. Although hexon protein comprises most of the capsid proteins, penton protein may be conserved in the IBH virus. Therefore, the penton gene can be utilized in next studies instead of the hexon gene.

کلیدواژه ها:

Inclusion Body Hepatitis (IBH) ، PCR ، Penton gene ، Iran ، Broiler ، Avian Adenovirus ، phylogenetic analysis.