Spasmolytic action of hydro alcoholic extract of Parsley (Petroselonium crispum) seed on rat's ileum contraction at presence histamine

Introduction: Traditional herbal medicine such as petroselinum crispum (parsly) has been used for treatment of intestinal disorders, Also, considering to the histamine receptors in the rat's ileum, the present study aimed to investigate the inhibitory effects of seeds of petroselinum crispum on the isolated rat's ileum at presence histamin. Materials and methods: In this study, 2 cm from the terminal isolated adult male rat's ileum Tyrode solution in an organ bath containing oxygenated (37 0c, PH=7.4) and placed under 1 gram tension contractions induced by 60mM potassium chloride and histamine concentrations (0.0001, 0.001, 0. 01, 0. 1mg/ml) and extract concentrations (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8 mg/ml) were added to the organ bath and their effects on the ileum's contractions were recorded by using Harvard oscilogrf. Results: The extract cumulative concentrations reduced ileum contraction induced by KCl in a dose-dependent manner (P<0/001). Spasmolytic effect of extracts (0.2mg/ml) after of the tissue exposure to histamine (0.1 mg/ml) as an agonist of the histaminergic system did not change. Conclusion: Although Parsley seed extract reduced rat's ileum contraction, it seems like histamine receptor are not involved in this activity.

کلیدواژه ها:

Histamin ، Petroselinum crispum ، Contraction ، Isolated ileum ، Rat