Comparison of alcoholic extract Nigella sativa L on the Viability of MCF-7 cancer cell line and Bone Marrow-Derived Stem Cells In Vitro

Breast cancer is the most frequent cancer among women, impacting 2.1 million women each year, and alsocauses the greatest number of cancer-related deaths among women .Nigella sativa L is an herbaceous, Orderof the Ranunculales and one-year-old plant. This plant has valuable components such as thymoquinone,which possesses anti-cancer properties. The aim of this study was evaluation and comparison of the effectof alcoholic extract Nigella sativa L on Breast cancer cells and normal Bone Marrow-Derived Stem Cells.Human Breast cancer cell line MCK-7 and normal Bone Marrow-Derived Stem Cells were cultured in vitroand treated with different concentrations of alcoholic extract Nigella sativa L for 24 hours. The MTT assaywas used to determine cell viability. Alcoholic extract Nigella sativa L in concentrations of 950 and 1250μg/ml had killed both of cells at 24 hours after treatment and caused morphological changes in cells whichwere more obviously in cancer cells. Alcoholic extract Nigella sativa L in concentrations of 650 μg/mlkilled 50 % cancer cells while that was little effect on Bone marrow stem cells. Cytotoxicity effect alcoholicextract Nigella sativa of on Breast cancer cells was more than its effect on normal Bone marrow stem cellsand can be regarded as a safe anticancer medicine.

کلیدواژه ها:

Cytotoxicity ، alcoholic extract Nigella sativa L ، Bone Marrow-Derived Stem Cells