Assessment of Immunomodulatory, Proliferative and of Crocin and Crocetin on Mesenchymal Stem Cells derived multiple sclerosis patients

Background: Multiple sclerosis (MS) is a chronic autoimmune disease of central nervous system (CNS) affecting more than 2 million people worldwide with extensive socioeconomic burdens Saffron (Crocus sativus L.) is a well-known spice with active pharmacologic components including crocin, crocetin, safranal and picrocrocin. Similar to crocin/ crocetin, mesenchymal stem cells (MSCs) have been shown to display immunomodulatory and anti-oxidant properties, which could be beneficial in treatment of various autoimmune disease. In the current study, we have evaluated the effects of crocin and crocetin on the functions of MSCs derived adipose tissue form multiple sclerosis.Methods: We used MTT assay to evaluate MSCs proliferation, and flow cytometry assay to measure the percentage of apoptotic MSCs and Tregs populations.Results: Our findings indicated that both crocin and crocetin at low concentrations (2.5, 5µM) exhibited significant effects on increasing MSCs viability and on protecting them against apoptosis-induced death. Moreover, increased Treg population was observed at lower doses.Conclusions: Altogether, both crocin and crocetin at lower concentrations exhibited more efficacies on MSCs with a better effect toward crocin. It seems that crocin and crocetin may be considered as complementary treatments for the MS patients who undergo MSCs transplantation.

کلیدواژه ها:

Immunomodulatory ، Multiple Sclerosis ، Mesenchymal stem cells.