Protective effects of alcoholic extract of Terminalia chebula on quinolinate-induced oxidative OLN-93 cell death

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 383

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شناسه ملی سند علمی:

MSC16_040

تاریخ نمایه سازی: 11 آذر 1398

چکیده مقاله:

Background: Quinolinic acid, is an endogenous metabolite of the kynurenine pathway and its pathologic accumulation has been found to induce neuroinflammatory and demyelinating diseases such as multiple sclerosis via excessive free radical’s generation. Terminalia chebula exhibited various pharmacological effects such as antioxidant, anti-inflammatory and neuroprotective properties. The aim of this study was evaluation of the protective effect of Terminalia chebula alcoholic extract (TCAE) on oxidative OLN-93 cells death induced by quinolinic acid.Methods: OLN-93 cells were pretreated with TCAE (6.25-50μg/mL) for 2h and then subjected to quinolinic acid (8mM) for 24h. Cell viability and the parameters of redox status including the levels of intracellular reactive oxygen species (ROS), and lipid peroxidation were measured using 2-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), 2,7-dicholorofluorecin diacetate (DCF-DA), and thiobarbituric acid assays, respectively. Results: Treatment with TCAE significantly increased cell viability following quinolinic acid insult at concentrations above 25μg/mL (p<0.01). Cytoprotective potential of TCAE also ameliorated ROS accumulation and lipid peroxidation induced by quinolinic acid.Conclusion: This work presents a novel insight into protection of oligodendrocytes against quinolinate toxicity by Terminalia chebula, suggesting its therapeutic potential against neurodegenerative and demyelinating diseases such as multiple sclerosis.

نویسندگان

Fatemeh Forouzanfar

Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences,Mashhad, Iran

Hamidreza Sadeghnia

Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center,Mashhad University of Medical Sciences, Mashhad, Iran

Roya Jamshidi

Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center,Mashhad University of Medical Sciences, Mashhad, Iran

Hassan Rakhshandeh

Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran