The Effect Of Recovered Overtrained Exercise On Mitogen-Stimulated And Non-Stimulated Splenocytes Response

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 395

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NASTARANCANSER02_208

تاریخ نمایه سازی: 22 دی 1396

چکیده مقاله:

It has been shown that the imbalanced immune system with poor Th1 and overactive Th2 responsescan result in a wide variety of chronic illness and cancer. In this study the effect of recoveredovertrained exercise on Th1/Th2 balance was evaluated in rat isolated splenocytes. Twelve maleWistar rats weighing 200-250g were randomly divided into two groups: Control (n=6) and recoveredovertrained (OR) (exercise plus 2 weeks recovery) (n=6). Exercised rats were summated to severrunning protocol (V=25m/min, 60min/day for 6 days a week) lasted 11 weeks (exercise boutsincreased in 8-11 weeks). At the end of the study; cell viability, proliferation, and interleukin 4 (IL-4) and interferon-γ (IFN-gmma) secretion in non-stimulated, concavaline A (Con A)-stimulatedsplenocytes were evaluated in both groups. Cell viability and proliferation of stimulated and nonstimulatedsplenocytes increased in the OR group compared to control group (p<0.01-p<0.001).There were not significant differences in IL-4 concentrations between non-stimulated splenocytesisolated from both groups. IL-4 concentration of Con A-stimulated cells supernatant in OR groupwas higher than control group (p<0.01). There were not significant differences in non-stimulatedand stimulated cell IFN-gmma concentration between groups. In non-stimulated cells, IFN-gmma/IL-4 ratio of OR group was not significantly different from control group. In ConA-stimulated cells,IFN-gmma/ IL-4 ratio was lower in exercise groups than control (p<0.01). The result showed thattwo weeks recovery restored Th1/Th2 balance, in non-stimulated splenocytes of overtrainedanimals. While in mitogen stimulation situation the susceptibility to chronic non-communicablediseases like cancer did not change.

نویسندگان

Zahra Gholamnezhad

Neurogenic Inflammation Research Centre, Mashhad University Of Medical Sciences, Mashhad, Iran Department Of Physiology, Faculty Of Medicine, Mashhad University Of Medical Sciences, Mashhad, Iran

Mohammad Hossein Boskabady

Neurogenic Inflammation Research Centre, Mashhad University Of Medical Sciences, Mashhad, Iran Department OfPhysiology, Faculty Of Medicine, Mashhad University Of Medical Sciences, Mashhad, Iran

Mahmoud Hosseini

Neurocognitive Research Centre, Mashhad University Of Medical Sciences, Mashhad, Iran