Evaluation of the cytotoxic capacity of silver nanoparticles synthesized from rubia tinctorum against human cancerous cell lines (mcf7)

Nanotechnology is a very broad approach with diverse applications in various fields, including biology and medicine. Recently, the biological synthesis of nanoparticles has attracted more attention thanphysical and chemical methods because of its biocompatibility and cost-effectiveness. The use of herbal extracts with multiple healing properties in nano synthesis is of particular importance. Various parameters such as surface area, particle size and surface reactivity determine the cytotoxicity of nanoparticles in plant extracts. In this study, the cytotoxic effects of green silver nanoparticle against breast cancer have been evaluated using the MTT assay.The malignant cells were seeded in 96 well plate after to achieve the logarithmic phase. After 24h of incubation, the cells were exposed to different concentrations (31.2, 62.5, 125, and 250 μg/ml) of nanoparticles. In order to evaluate the effect of time on the toxicity induced by the nanoparticle, 24, 48 and 72 h after treatment, the toxicity was evaluatedusing MTT. After incubation, the treatment medium was evacuated and the MTT solution was added to each of the wells for 4 h. Then, in order to dissolve the formazan crystals, 100 μl DMSO was addedto each well. Finally, the optical absorption at 570 nm was evaluated by (Epoch, USA) spectrophotometer. Invert microscope was used to observe morphological alterations of the MCF-7 cells during treatment.The results showed that the biosynthesized nanoparticle is able to inhibit cancerous cells with high potency, and this suppression is dependent on the dose and the time of the treatment with IC50 about (40 μg/ml). Microscopic studies showed significant changes in the shape and size of treated cells, such as cytoplasmic sprouts, decrease of cell volume and cell shrinkage.The current investigation suggest that nanoscale particles may be a novel potential adjuvant chemotherapeutic agent to suppress breast cancer

کلیدواژه ها:

Breast Cancer ، Cell and Cancer ، Cancer Genetics ، Targeted Cancer Therapy ، Drugs and Cancer