Effect of caralluma tuberculata hydroalcoholic extract on cell viability of pc3 prostate cancer cell line

A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. Caralluma tuberculata containing different components such as Flavonoidswhich have shown anti-cancer benefits. The aim of present study was to determine the anti- proliferative potential of the hydroalcoholic Caralluma tuberculate extract (HACE) on PC-3 human Prostate carcinoma cell line. PC-3 cells were grown in RPMI-1640 medium as monolayer cultures grown at 37°C in a humidified atmosphere of 5% Co2 in air. the anti-proliferative activity of the HACE was evaluated by MTT assay 24h,48h and 72h after exposure. The reaction is carried out in situ in multiwell plates, and the reaction product, a purple-colored formazan soluble in dimethylsulfoxide, is measured colorimetri-cally, using a multiwell plate reader in 630 nm. The relationship between the number of cells per well and the OD of the formazan produced was linear up to 3 x 105 cells per well. The mean OD at day 2 for 24 samples plated at 3 x 103 cells per well was 0.348±0.10, range 0.085- 0.688. the results of MTT showed that ED50 of PC-3 is 150 μg/ml of the HACE after 48h of treatment. The MTT test is based on the enzymatic reduction of the tetrazolium salt MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide] in living, metabolically active cells but not in dead cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. this study found that the HACE induced cell death in PC-3 human prostate carcinoma cell line.

کلیدواژه ها:

Prostate Cancer ، Cancer Prevention ، Cell and Cancer ، Solid Tumors ، Cancer Treatment and Management ، Drugs and Cancer