Evaluation of cytotoxic effects of silver nanoparticle synthetized using brassica napus on cancerous cells

Liver cancer, such as other cancers, occurs due to a mutation and proliferation, or during apoptosis reduction. Different treatments have been used to control liver cancer, but the most common problemfor treatment of many cancers is the effect of treatment on normal cells. Therefore, in laboratory studies, the effect of a substance in addition to cancer cells on normal cells should also be evaluated. Insome studies, umbilical cord cells are used as normal cells to evaluation the toxicity of the various substance. Today, nanoparticles is the one of the materials that has attracted much attention. The effectof these materials on normal and cancerous cells has been evaluated in many studies. The size, shape and amount of crystallization of nanoparticles can play an important role in their biological properties.These materials have different uses in treatment and medicine. In this study, the cytotoxic effects of silver nanoparticles on umbilical cord cells as normal cells and HepG2 as cancer cells 24 and 48 h afterexposure was studied. Inhibition of cells under silver nanoparticle treatment: In this method, the MTT solution is used to examine the viability of normal and cancer cells. The basis of the reaction is thetetrazolium salt regeneration by the enzyme present in the mitochondria of the living cells. As a result of regeneration, insoluble crystals of formazan are formed. These crystals are dissolved in DMSO andcreate a violet color. Therefore, the amount of survival of the cells estimated by absorption rate. For this purpose, the cells were seeded and then treated for various time, finally, with adding MTT andfollowed by DMSO adding, the absorbance was recorded at 570 nm. Morphological changes in treated cells were compared with untreated using inverted microscope. The cytotoxic capacity of nanoparticlesagainst liver cells is 24 and 48 hours after concentration-dependent treatment with IC50 about 0.2 μg/ml at 24 and 48 h after treatment. Time of exposure did not have much effect on toxicity. Investigating the cytotoxic capacity of nanoparticles on umbilical cord cells showed that about 50% of the cells were inhibited in 1 and 0.7μg/ml concentrations of nanoparticle after 24 and 48h respectively.The cytotoxicity of nanoparticles on the cells depends on the concentration and time of treatment. The study of morphological changes of treated cells compared to untreated showed that the cells weredeformed on both cell lines reviewed. The size and volume of the cells decreased and the cells were wrinkled. The fragmentation of the nucleus and cells was seen as an other changes. In this study, thecytotoxic effect of nanoparticle on HepG2 and Huvec was evaluated and the results showed that the nanoparticle inhibited cancer cells more potently than normal cells, and perhaps this substance couldbe used to treat liver cancer.

کلیدواژه ها:

Cancer Prevention ، Cancer Stem Cells ، Stem Cells and Cancer ، Cancer Treatment and Management ، Drugs and Cancer