Free radical scavenging property and cytotoxic effect of zinc oxide nanoparticles synthesis using ceratonia siliqua l extract

Antioxidants are commonly found in many foods and can keep the cells from free radical invasion and oxidative stress caused by them. Free radicals in oxidation process damage to cells and leads to various diseases, including degenerative diseases and cancer. Antioxidants, by inhibiting free radicals, minimize the damage to the cells and prevent and control the progression of diseases such as cancer. Therefore, the use of antioxidant substances can save cells from many injuries. Some materials, including nanoparticles, have cytotoxic and antioxidant properties that can be used in order to inhibit andsuppress cancer cells. In this study, the antioxidant capacity and cytotoxic property of Zinc Oxide Nanoparticles Synthesis Using Ceratonia siliqua L (ZnO-NPs/Cs ) were estimated. The HT-29 cancercells were exposed to the doses prepared from the serial dilution of ZnO-NPs/Cs for 24 hours and then the cell survival percentage was determined by adding the MTT (for 4h) and DMSO solutions anthen measuring the absorbance. The simplicity and consistency of DPPH free radicals and the high speed of ABTS free radicals they has become one of the most widely methods that used for antioxidantcapacity measurement. In both methods, at first, free radicals are prepared according to a specific protocol, in the following, the equal volume of the various doses of ZnO-NPs/Cs and ABTS , DPPH free radicals are combined. After the incubation time, the absorbance of the samples was measured. The results of the toxicity assay showed that the ZnO-NPs/Cs suppresses HT-29 cancer cells withIC50 about 250 μg/ml. The viability of HT cells at 500 μg/ml decreased to about 24.43% . Survey results showed that with increasing the doses of the ZnO-NPs/Cs, increases its anti-radical effect. Also,IC50 index of ZnO-NPs/Cs for ABTS was about 500 μg/ml while in this concentration, the DPPH free radical inhibition is about 28.9%. It should be noted that IC50 has an inverse relationship with antioxidant activity of NPs. The results indicate that ZnO-NPs/Cs with further testing and final approval can be used to treat cancer because its antioxidant effects and the cytotoxic effects on cancer cells.

کلیدواژه ها:

Breast Cancer ، Gene and Cancer ، Cell and Cancer ، Cancer Treatment and Management ، Drugs and Cancer