The effects of cytotoxic dose of estradiol valerate on caspase 8 activity level in colon cancer (ht29) cells

The HT-29 cell line is the most commonly used colorectal adenocarcinoma cell line with epithelial morphology, which is used in many cellular studies. These cells were first produced in 1964 for 44-year-old Caucasian women with colon adenocarcinoma. HT29 cells under the standard conditions have an unpolarized growth, and they form undifferentiated cell lines that provide a good, To investigate theeffects of various factors on these cells. By creating mutations in the intestinal tract, carcinogenic signaling pathways are activated in the cells, some of which play an important role in the developmentof cancer. Caspase-8 is a member of the cysteine proteases, which are implicated in apoptosis and cytokine processing. Cytotoxic concentrations (1mg/ml) of Estradiol valerate was used in our study.Cells were grown and incubated in standard situation. Analyses were conducted using the SPSS 20 and ANOVA. Our results indicated that exposure to 1mg/ml of estradiol valerate led to significant increase in caspe 8 activity compared to control cells. In our study we found that exposure of colon cancer cells to cytotoxic dose of estradiol valerate led to increased activity of caspase 8 enzyme indicating that apoptosis occurred through internal pathway in which caspase 8 is activated. In a study conducted by Levinteir et al. In 1992 on the study of the effects of steroid hormones on the proliferation of human colon cancer cells in cell culture media, the results showed that the analogue of estrogen and ethinyl progesterone could inhibit the growth of colon carcinoma cells. In our study we found that exposure of colon cancer cells to cytotoxic dose of estradiol valerate led to increased activity of caspases 8 enzyme indicating that apoptosis occurred through internal pathway in which caspe 8 is activated

کلیدواژه ها:

Colon Cancer ، Cancer Diagnosis ، Cell and Cancer ، Cancer Treatment and Management ، Drugs and Cancer