Somatic emberyogenesis and plantlet regeneration in Crocus speciosus

Somatic embryogenesis was induced from shoot meristems of Crocus speciosus. The explants were cultivated at 20 °C in darkness on Linsmaier and Skoog medium supplemented with 3% (w/v) sucrose and different growth regulator combinations. The best embryogenic rates were obtained in the presence of 4 mg/l kinetin and 1 mg/l 2,4-D, as approximately 30 % of callus formed on this medium produced somatic embryos. All phases of somatic embryogenesis were observed on half strength MS medium with 1 mg/l ABA. Maturated embryos germinated in the presence of 25 mg/l GA3. Conversion of bipolar embryos to plantlets was obtained when they were transferred to half strength Murashige and Skoog medium supplemented with 1 mg/l IAA and 1 mg/l kinetin at 20 °C under a 16/8-h (light/dark) photoperiod.

کلیدواژه ها:

Crocus speciosus ، plant regeneration ، somatic embryogenesis