Effects of salinity on growth and nitrogen fixation of two cultivars of alfalfa inoculatel by different strains of Sinorhizobium meliloti

سال انتشار: 1384
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 1,855

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شناسه ملی سند علمی:

NBCI04_396

تاریخ نمایه سازی: 30 دی 1386

چکیده مقاله:

Effects of salinity (sodium chloride ) on growth and biological nitrogen fixation (BNF) was investigated in roots of alfalfa (Bami and American cultivars) inoculated by six different strains of Sinorhizobium bacteria.These strains included a wild type named S.meliloti wild type ( S), two mutated strains S.meliloti nifK-(S) and 392259S. meliloti nifH-( S237), which were without plasmid and three strains W.T( pSRK9) , nifK-(pSRK9) and nifH-(pSRK9), with plasmid. (pSRK9) plasmid carried nitrogen fixation system of Agrobacterium tumefaciens. Salinity treatments were 0, 50, 100 mM sodium chloride. Results showed that dry weight production in those plants which were treated with 0,50 mM sodium chloride were the same. Mean comparison for active nodule number, dry weight of whole plant, sugar content of root and nitrogenase activity showed that both cultivars of alfalfa inoculated with nifK-(pSRK9) strain was much higher than others. In the other hand inoculation of alfalfa plants with high salinity tolerant of Sinorhizobium meliloti bacteria which were transferred with the plasmid contain the genes that are able to fix nitrogen can improve plant production under salinity.

نویسندگان

M Motahari

Biology Department, Faculty of Sciences, Shahid Bahonar University of Kerman, Iran

KH.M Kalantari

International Centre of Sciences and High Technology and Environmental Sciences, Mahan, Kerman