Molecular detection of host cytokine expression in cancer and non cancer Helicobacter pylori infected parients cia Semi-quabtitative RT-PCR

Helicobacter pylori (Hp) is a gram negative, spiral shaped bacterium that is recognized as the major agent of chronis gastritis. Infection with Hp plays an important role in the pathogenesis of peptic ulcer and is a risk factor for the development of adenocarcinoma and primary gastric lymphoma.Cell-mediated immune responses have a decisive role in the outcomes of most bacterial infection thus far studied. Not an exception to the rule is Hp infection which is believed to be associated with a Th1 type of immune responses to the degree that is considered as an immunopathogenic disease. The aim of this study was to determine the level IFN-γ (proinflammatory) and IL-4 (anti-inflammatory) cytokine expression as indicator of Th1 and Th2 immune responses in gastric cancer (GC) and non gastric cancer dyspeptic (Non GC) specimens from Iranian patients by gene specific RT-PCR to detect low levels of specific mRNA and allow the quantification of cytokine expression from very small specimens. This study has pursued the role of these cytokines as indicators of degree of risk prognosis for the Iranian infected patients. We hereby, report the detection of gastric cytokines in Hp infected patients by gene-specific semi-quantitative RT-PCR.

کلیدواژه ها:

cytokine ، IL-4 ، IFN-م ، RT-PCR ، cDNA ، immune response Th1 ، Th2