Kinetic Study of Trypsin Enzymatic Effect on Digestion of Refolded Recombinant Insulin by HPLC, Elisa, Non-Denaturing and Tris-Tricin PAGE Methods

An analytical scheme for monitoring recombinant human insulin digestion is suggested. The scheme includes high Performance Liquid Chromatography, Non-Denaturing and Tris-Tricin PAGE and Elisa .The use of this scheme at all digestion steps provided optimization of certain parameters (conditions for fusion protein FP duration of cleavage by trypsin. The proposed scheme may be used for solving various problems in digestion of other recombinant proteins.