Leishmania Sp. DNA detecting in cutaneous paraffin embedded block by a novel hemi-nested polymerase chain reaction method

To investigate Leishmania Sp. DNA in cutaneous paraffin embedded blocks, a hemi-nested PCR method was developed and tested five cutaneous paraffin blokes which suspected to Leishmania Sp. infection. DNA form paraffin blocks were extracted by DNP™ DNA Extraction kit and then subjected for PCR and sequencing. An internal control was carried out that determined human factor XIII genome to investigate accuracy of DNA extraction. Three of five samples showed positive Leishmania DNA amplification. Results of sequencing showed the Leishmania genome sequences in all three positive samples. In conclusion, the developed hemi-nested PCR method can readily detect Leishmania DNA in the paraffin embedded samples that are suspected to be infected with Leishmania.

کلیدواژه ها:

hemi-nested PCR ، Leishmania ، paraffin embedded block