Antibiotic resistance pattern and expression of type III secretion system gene (ExoU Exotoxin) by real time –PCR among pseudomonas aeruginosa isolated from patients with burn wounds in Velayat burn center, Rasht, Iran

Background and Aim : Pseudomonas aeruginosa is an opportunistic pathogen that is widely distributed in nature and is often found in hospital wet environments. Due to limited nutritional requirements and resistance against antimicrobial agents, it seems one of the most significant pathogen acquired at hospital. Antibiotic resistance in Pseudomonas aeruginosa is the main reason to conduct an overt and aptly designed study and research. This study evaluates the antibiotic resistance and gene expression of the type 3 secretion system (exoU). Methods : In this study 100 samples of Pseudomonas aeruginosa were isolated from hospitalized patients in Velayat Hospital of Rasht. Antibiotic resistance to 8 antibiotics (Gentamicin, Amikacin, Tobramycin, Ceftazidime, Piperacillin, Carbencililin, Ciprofloxacin and Imipenem) was assessed after confirmation of isolates and purification. Also, type 3 secretion system (exoU) gene expression was determined by Real-Time PCR. Results : Resistances to antibiotics out of isolated species were: Piperacillin (50%), Carbenicillin (81%), Ampiclavin (22%), Ciprofloxacin (49%), Gentamicin (54%), Amikacin (24%), Tobramycin (60%), Sepharimide (25%) multi-drug resistant isolates (35%) and Aminoglycosides (16%). ExoU expression in Pseudomonas strains isolated from burn wounds was 41% and there was a significant relationship between expression of ExoU gene and resistance to ciprofloxacin, gentamicin, and tobramycin antibiotics (P = 0.001). Conclusion : The results indicate that Imipenem is the most effective antibiotic and ExoU exotoxin gene has a significant effect on the three antibiotic resistance of the type 3 secretion system. Considering that ExoU is one of the most important proteins of the type 3 secretion system in pathogenesis and pathogenicity as well as antibiotic resistance, the necessity of studying on this protein plays an important role in the identification of strains isolated from burn wounds and its relation with antibiotic resistance.

کلیدواژه ها:

Pseudomonas aeruginosa ، Real-Time PCR ، antibiotic resistance ، gene expression