Production of exopolygalacturonase from wheat flour by Aspergillus awamori in submerged and surface culture fermentation

Pectinases are a grup of hydrolytic enzymes that degrade pectic substances. They are produced by a variety of microorganisms including filamentous fungi. Studies were carried out on the production of exoplygalacturonase (exo-PGase) using wheat flour by Aspergillus awamori in submerged fermentation (SmF) and surface culture fermentation (SCF). Higher titres of exopolygalacturonase was observed when medium was supplements with carbon (4g/L Pectin for both SmF and SCF) and nitrogen (ammonium sulphate 6g/L for both SmF and SCF) sources. SCF proved to be a better process than SmF for exopolygalacturonase production, giving the highest enzyme production rate in 96h. Exo-PGase produced by Aspergillus awamori presented optimum activity at pH 5 for SmF and 5.5 for SCF. Using of continuous system for exo-PGase production was obtained better results than batch system.

کلیدواژه ها:

Exopolygalacturonase ، Aspergillus awamori ، Wheat flour ، Surface culture fermentation ، Submerged fermentation ، Continuous production