Isolation And Identification Of Bacillus subtilis From Soil: : Evaluation Seven Characterization For Probiotic Usage

Health promoting microorganisms, probiotics, have been recently increasingly included in various food products and proposed for use as a food supplement or as a therapy for several infectious diseases. We studied the safety of one bacillus subtilis that isolate from Soil saturated salt lake Qom and after isolation and identification bacteria we studied the safety of this Bacillus strain. Methods In this research, we assessed characteristics that are associated with safety of this Bacillus as a probiotic: Soil sampling was done first and then transferred to laboratory and phenotypic and genotypic tests were performed .After 16S Rdna sequencing, the safety studies were performed in bacteria for using as a probiotic. Result 16S Rdna revealed that this bacteria is B. subtilis . The strain tested were found to be sensitive to most of the antibiotics used .The strain was non-hemolytic, negative lecithinase and negative enterotoxin gene. The strain was shown to produce surfactin and biofilm. This isolate showed autoaggregation and had hydrophobicity in the xylene, chloroform and ethyl acetate. Spores were shown to be completely resistance to gastrointestinal tract (GIT) conditions and the strain produce antimicrobial activity using a panel of Gram-positive and negative bacteria. Discussion The surfactin that produced could facilitate entry into cells by rupturing the phospholipid membrane. Biofilm-producing have been shown to remain bacteria in the GIT. Spores were shown to be completely resistance to exposure GIT. This is important as it ensures that the stated dose actually reaches the region of the GIT where it should exert its effect. Hydrophobicity and autoaggregation of the cell increases the level of adhesion and colonization on tissues. An important aspect of safety assessments for potentialmicrobial food supplements is to ensure that no erotoxins are produced by the bacterium and this bacteria didn'show that and no virulence factors like haemolysis and lecithinase are produced