In Silico Analysis Of Antimicrobial Peptide Cecropin Fused With Human Lysozyme With Splicing By Overlap Extension PCR(SOEING PCR)

Genetic manipulation of bacteria often requires the joining together of more than one DNA segment to form a hybrid DNA. Overlap extension is a sequence-independent method for site-directed mutagenesis and recombination of DNA molecules. Lysozyme is an abundant, cationic antimicrobial protein that plays an important role in host defense. lysozyme alone is inactive against Gram-negative bacteria because it cannot reach the peptidoglycan layer. Cecropins are cationic molecules with a wide range of antimicrobial activities.